Reference plate for checking photometric and wavelength accuracy

With the reference plate 666R113 from Hellma Analytics the photometric and wavelength accuracy of microplate readers can be checked.

The reference plate has the same dimensions as a microplate with 96 wells and a 6.6 mm diameter per window (H 13.0 x D 127 x L 85.5 mm).

Each of the four neutral density glasses used (columns 3 – 10) can measure the absorbance value for 16 windows. The Holmium glass (column 11+12) is used to check the wavelength accuracy in 16 windows while a further 16 windows (columns 1+2) do not contain glass and serve as references.

Note

The reference plate consists of four neutral density glasses with different nominal absorbance values. Therefore the linearity of the absorbance scale can be checked by plotting the absorbance values for each wavelength in a diagram against the measuring values on the calibration certificate.

 

Article-No. 666R113  Request this article
Application Reference plate for microplate readers for checking the photometric and wavelength accuracy
Content Column 1+2: empty - reference
Column 3+4: ND with 0.5 Abs
Column 5+6: ND with 1.0 Abs
Column 7+8: ND with 1.5 Abs
Column 9+10: ND with 2.0 Abs
Column 11+12: Holmium glass
Standard Certification Photometric accuracy certified with DAkkS calibration certificate at
Wavelengths: 405, 450, 490, 650 nm; at 8 Points in a row
Slit width: 1 nm;
Wavelength accuracy certified with DAkkS calibration certificate at
Wavelengths: 279, 361, 453, 536, 638 nm
Slit width: 1 nm
Possible certification Photometric accuracy possible at:
Wavelengths: all possible between 405 bis 800 nm,
Slit widths: all up to 2 nm possible;
Wavelength accuracy possible at:
Wavelengths: 279, 287, 361, 418, 445, 453, 460, 536, 638 nm
Slit width: 1 nm up to 2 nm

 

Helpful tool for monitoring mean and target values

Control charts, such as e.g. target value charts are used to graphically display measured values. Here, the value specified in the Hellma Analytics calibration certificate is used as the target value. All measured values must be within the range of the measurement uncertainty, otherwise there is an out-of-control situation.

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Wavelength Accuracy UV5, F1, F7 (Control Charts EN)

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Photometric Accuracy F2, F3, F4 (Control Charts EN)

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Optical Reference Materials (Brochure & User Manual EN)

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